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Updated: Jun 12, 2026

Collection of Human Follicular Fluid, Follicle Somatic Cells, and Immature Oocytes from Individuals Undergoing In Vitro Fertilization
Published on: October 24, 2025
Molecular profiling of human oocytes after vitrification strongly suggests that they are biologically comparable with
Cinzia Di Pietro1, Marilena Vento, Maria Rosa Guglielmino
1Dipartimento di Scienze Biomediche, Sezione di Biologia Generale, Biologia Cellulare, Genetica molecolare G. Sichel, Università degli Studi di Catania, Catania, Italy. dipietro@unict.it
Abstract:
To assess the effects of vitrification on the biomolecular profile of oocytes, we analyzed through real-time reverse transcriptase-polymerase chain reaction eight genes encoding critically important proteins for embryo development and compared this partial transcriptome with that of freshly collected gametes isolated from the same women. The comparison of the molecular profiles demonstrated that our vitrification protocol does not alter the biomolecular quality of oocytes: in fact, between the two groups we found the absence of statistically significant variations. Accordingly, this cryopreservation technique might be helpful in preserving women's fertility.
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