Related Experiment Video
Updated: Jun 12, 2026

An Approach to Constructing Multispecies Biofilm Communities from Rhizosphere Soil
Published on: May 24, 2024
Paenibacillus tianmuensis sp. nov., isolated from soil
Xuechang Wu1, Haihuan Fang1, Chaodong Qian1
1Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, PR China.
Abstract:
Two closely related, Gram-stain-negative, rod-shaped, spore-forming strains, B27(T) and F6-B70, were isolated from soil samples of Tianmu Mountain National Natural Reserve in Zhejiang, China. Phylogenetic analysis based on 16S rRNA gene and rpoB sequences indicated that the isolates were members of the genus Paenibacillus. Both isolates were closely related to Paenibacillus ehimensis IFO 15659(T), Paenibacillus elgii SD17(T) and Paenibacillus koreensis YC300(T) (≥ 95.2 % 16S rRNA gene sequence similarity). DNA-DNA relatedness between strain B27(T) and P. ehimensis DSM 11029(T), P. elgii NBRC 100335(T) and P. koreensis KCTC 2393(T) was 21.2, 28.6 and 16.8 %, respectively. The major cellular fatty acids of strains B27(T) and F6-B70 were anteiso-C(15 : 0) and iso-C(15 : 0). The cell wall contained meso-diaminopimelic acid. The two isolates differed from their closest neighbours in terms of phenotypic characteristics and cellular fatty acid profiles (such as variable for oxidase, negative for methyl red test, unable to produce acid from d-fructose and glycogen and relatively higher amounts of iso-C(15 : 0) and lower amounts of C(16 : 0) and iso-C(16 : 0)). Strains B27(T) and F6-B70 represent a novel species of the genus Paenibacillus, for which the name Paenibacillus tianmuensis sp. nov. is proposed. The type strain is B27(T) ( = DSM 22342(T) = CGMCC 1.8946(T)).
Related Concept Videos
Bacterial Phylum Actinobacteria
Bacterial Phylum Bacteroidota
Microbiota of the Large Intestine
Other Unique Bacteria
Bacterial Phylum Spirochaetes
Tetanus
