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Updated: Jun 12, 2026

Automated Counterflow Centrifugal System for Small-Scale Cell Processing
Published on: December 12, 2019
Novel Design for Centrifugal Counter-Current Chromatography: III. Saw Tooth Column
Yi Yang1, Haji Akber Aisa, Yoichiro Ito
1Bioseparation Technology Laboratory, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 10 Center Drive, Building 10, Room 8N230, Bethesda, MD 20892, USA.
Abstract:
The toroidal coil using an equilateral triangular core and zigzag pattern column have improved both retention of the stationary phase and peak resolution of the conventional toroidal coil in centrifugal counter-current chromatography. To further improve the retention of stationary phase and peak resolution, a novel saw tooth column was designed and the performance of the system was evaluated at various flow rates. The results indicated that both retention of the stationary phase and peak resolution were improved as the flow rate was decreased and at a flow rate of 0.005 ml/min the resolution is remarkably increased. Modification of the tubing called flat-twisted tubing further improved the peak resolution without increasing the column pressure. With a decreased column length at a capacity of about 0.2 ml, resolution of the saw tooth column was 1.02.
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