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[Effect of Dureping injection on TIR signal pathway on Ana-1 cells]
Yanli Zhang1, Ningping Wang, Ligang Gu
1Department of Etiology and Immunology of Ningxia Medical University, Yinchuan 750004, China.
Objective:
To investigate the influence of Dureping injection to the murinal celiac macrophage Ana-1 on TIR signal pathway.
Method:
Ana-1 cell line was infected by influenza virus FM1 strain and treated with the Dureping injection in different concentrations (10.1 mg x L(-1) group) for 12 h and 24 h. Then we collected the cells, extracted mRNA and measured the expressions of TLR7, MyD88, IRAK4, TRAF6 and NF-kappaB p65 respectively by RT-PCR.
Result:
Dureping injection down-regulated the expression of TLR7, MyD88, IRAK4, TRAF6 and NF-kappaB p65 mRNA in Ana-1 cell line infected by influenza virus, in a dose-dependent manner significantly.
Conclusion:
Dureping injection has an obvious effect against influenza virus FM1 strain by regulating the TIR signal pathway.
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