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Characteristics of [3H]ryanodine binding to the rabbit cerebral microsomes
1Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Brain Research
|February 1, 1991
Abstract:
Specific binding of [3H]ryanodine to the rabbit cerebral microsomes was dependent on free Ca2+ at micromolar concentrations and significantly increased by AMP-PNP and caffeine. Scatchard analysis showed a high and a low affinity binding site. The results suggest the presence of ryanodine binding sites which are activated by Ca2+ but with low efficacy, and greatly modified by adenine nucleotide, Mg2+ and also by caffeine.