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Generation of single-chain Fvs against detergent-solubilized recombinant antigens with a simple coating procedure

Torahiko Tanaka1, Yuichiro Hasegawa, Makoto Saito

  • 1Department of Advanced Medical Science, Nihon University School of Medicine, 30-1 Oyaguchi-kamimachi, Itabashi, Tokyo 173-8610, Japan. tanakat@med.nihon-u.ac.jp

Journal of Bioscience and Bioengineering
|June 16, 2010
PubMed

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Detergent Purification of Membrane Proteins01:18

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Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...