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Related Concept Videos

UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
UV–Vis Spectroscopy: Beer–Lambert Law01:09

UV–Vis Spectroscopy: Beer–Lambert Law

The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The modern...
UV–Vis Spectroscopy: Woodward–Fieser Rules01:29

UV–Vis Spectroscopy: Woodward–Fieser Rules

UV–Visible absorption spectra of conjugated dienes arise from the lowest energy π → π* transitions. The light-absorbing part of the molecule is called the chromophore, and the substituents directly attached to the chromophore are called auxochromes. A strong correlation exists between the absorption maxima, λmax, and the structure of a conjugated π system. The Woodward–Fieser rules predict the value of λmax for a given structure by adding the contributions...
Calibration Curves: Linear Least Squares01:20

Calibration Curves: Linear Least Squares

A calibration curve is a plot of the instrument's response against a series of known concentrations of a substance. This curve is used to set the instrument response levels, using the substance and its concentrations as standards. Alternatively, or additionally, an equation is fitted to the calibration curve plot and subsequently used to calculate the unknown concentrations of other samples reliably.
For data that follow a straight line, the standard method for fitting is the linear...
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for electronic transitions. As a result...

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Related Experiment Video

Updated: Jun 12, 2026

ARL Spectral Fitting as an Application to Augment Spectral Data via Franck-Condon Lineshape Analysis and Color Analysis
07:11

ARL Spectral Fitting as an Application to Augment Spectral Data via Franck-Condon Lineshape Analysis and Color Analysis

Published on: August 19, 2021

Reliable and efficient program for fitting Galatry and Voigt profiles to spectral data on multiple lines.

X Ouyang, P L Varghese

    Applied Optics
    |June 16, 2010
    PubMed
    Summary

    This study presents an efficient program for fitting experimental spectral line data to Galatry and Voigt profiles using least-squares analysis. The developed algorithm offers a robust method for analyzing complex spectral line shapes.

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    ARL Spectral Fitting as an Application to Augment Spectral Data via Franck-Condon Lineshape Analysis and Color Analysis
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    Area of Science:

    • Spectroscopy
    • Computational Physics
    • Data Analysis

    Background:

    • Analyzing experimental spectral data with overlapping lines requires accurate line shape models.
    • Existing methods may lack efficiency or flexibility for complex spectral profiles.

    Purpose of the Study:

    • To develop and present an efficient computational program for spectral line shape fitting.
    • To implement least-squares fitting for Galatry and Voigt profiles.

    Main Methods:

    • Least-squares fitting algorithm.
    • Implementation of Galatry and Voigt line shape models.
    • Detailed algorithm and program design considerations.

    Main Results:

    • An efficient program for fitting experimental spectral data to theoretical line shapes.
    • Demonstration of the program's utility with examples.
    • The procedure's applicability to more complex line shape profiles.

    Conclusions:

    • The developed program provides an efficient solution for spectral line shape analysis.
    • The methodology is adaptable for fitting various complex spectral line profiles.