Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 12, 2026

Building Up Skin Models for Numerous Applications - from Two-Dimensional (2D) Monoculture to Three-Dimensional (3D) Multiculture
08:32

Building Up Skin Models for Numerous Applications - from Two-Dimensional (2D) Monoculture to Three-Dimensional (3D) Multiculture

Published on: October 20, 2023

A novel dermal tissue construct: development and in vitro characterization.

Manisha S Deshpande1, Pushpa V Kuchroo

  • 1Reliance Life Sciences Pvt. Ltd., Dhirubhai Ambani Life Sciences Center, R-282, TTC Area of MIDC, Thane-Belapur Road, Rabale, Navi Mumbai, Maharashtra 400701, India. manisha_deshpande@relbio.com

Biotechnology Progress
|June 16, 2010
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A decoy strategy to activate the immune system.

IUBMB life·2021
Same journal

Advanced glucose control strategies leveraging Raman spectroscopy for optimized mammalian cell culture manufacturing.

Biotechnology progress·2026
Same journal

Mechanistic deconvolution of BSA size variants by constrained Raman pseudo-Voigt hard modeling during anion-exchange chromatography.

Biotechnology progress·2026
Same journal

Status and future of recombinant adeno-associated virus vector manufacturing.

Biotechnology progress·2026
Same journal

Multifaceted algae as an ingredient in alternative meat formulations.

Biotechnology progress·2026
Same journal

In-line Raman spectroscopy real-time glucose prediction method for commercial pneumococcal vaccine drug substance fermentation manufacturing process control.

Biotechnology progress·2026
Same journal

Prolonged autophagy induction correlates with host cell protein reduction in CHO cell culture.

Biotechnology progress·2026
See all related articles

A novel dermal tissue construct using human fibroblasts and chitosan effectively promotes healing in diabetic nonhealing ulcers. This innovative scaffold enhances growth factor delivery and cell viability for accelerated wound repair.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Diabetic nonhealing ulcers represent a significant clinical challenge.
  • Current treatments often face limitations in promoting effective wound closure.
  • Accelerated healing strategies are crucial for managing diabetic foot complications.

Purpose of the Study:

  • To develop and characterize a novel dermal tissue construct for treating diabetic nonhealing ulcers.
  • To evaluate the construct's potential to enhance growth factor diffusion and cell-mediated healing.
  • To assess the construct's biocompatibility, cell viability, and growth factor production.

Main Methods:

  • Fabrication of a dermal tissue construct using human dermal fibroblasts and a chitosan sponge.

More Related Videos

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding
08:35

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

Published on: February 26, 2015

Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation
09:30

Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation

Published on: March 4, 2016

Related Experiment Videos

Last Updated: Jun 12, 2026

Building Up Skin Models for Numerous Applications - from Two-Dimensional (2D) Monoculture to Three-Dimensional (3D) Multiculture
08:32

Building Up Skin Models for Numerous Applications - from Two-Dimensional (2D) Monoculture to Three-Dimensional (3D) Multiculture

Published on: October 20, 2023

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding
08:35

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

Published on: February 26, 2015

Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation
09:30

Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation

Published on: March 4, 2016

  • Arrangement of fibroblasts in a 3D sheet adhered to the chitosan sponge for optimized growth factor delivery.
  • Assessment of cell viability, mechanical properties, and flexibility of the construct.
  • Quantification of vascular endothelial growth factor (VEGF) and Interleukin-8 (IL-8) production.

    • Main Results:

    • The construct demonstrated high cell viability (>90%) and significant production of VEGF (42-31 ng/24h) and IL-8 (375-1065 ng/24h).
    • The unique design facilitates direct diffusion of growth factors to the wound bed, bypassing matrix impedance.
    • The porous, flexible chitosan sponge ensures gas exchange and conforms to wound contours while maintaining structural integrity.

    Conclusions:

    • The developed dermal tissue construct shows promise as an effective therapeutic agent for diabetic nonhealing ulcers.
    • Its design optimizes the delivery of key healing factors, supporting accelerated wound regeneration.
    • Further clinical studies are warranted to validate its efficacy in patients with diabetic ulcers.