Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical significance of high-density lipoprotein cholesterol and its dynamic change in patients with lymphoma-associated hemophagocytic lymphohistiocytosis.

Therapeutic advances in medical oncology·2026
Same author

Quasi-Discrete Channels of Porous Coordination Polymers for Selective Multiscenario CO<sub>2</sub> Recognition.

ACS applied materials & interfaces·2026
Same author

Application of orthodontic elastics for the treatment of midline diastemas.

General dentistry·2026
Same author

Narrow-Bandgap Donor-Acceptor Polymers for Efficient Solar Water Evaporation and Thermoelectric Power Generation.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Laser-Assisted Diamond Cutting for Low-Damage Fabrication of High-Q CaF<sub>2</sub> Whispering-Gallery Mode Resonators.

Micromachines·2026
Same author

Severity-Dependent Association Between Allergic Rhinitis and Adenoid Hypertrophy in Dust Mite-Sensitized Children from Northern China: A Cross-Sectional Analysis of 170 Cases.

Journal of asthma and allergy·2026

Related Experiment Video

Updated: Jun 12, 2026

Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture
11:00

Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture

Published on: August 8, 2013

Quantifying mRNA synthesis and decay rates using small RNAs.

Vlad Elgart1, Tao Jia, Rahul Kulkarni

  • 1Department of Physics, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA. velgart@stanford.edu

Biophysical Journal
|June 17, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces a novel method to simultaneously measure messenger RNA (mRNA) transcription and decay rates. This approach utilizes small regulatory RNAs (sRNAs) to precisely control mRNA lifetimes, offering a more accurate understanding of gene expression regulation.

More Related Videos

Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation
09:59

Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation

Published on: May 3, 2018

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains
12:21

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains

Published on: December 13, 2014

Related Experiment Videos

Last Updated: Jun 12, 2026

Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture
11:00

Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture

Published on: August 8, 2013

Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation
09:59

Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation

Published on: May 3, 2018

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains
12:21

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains

Published on: December 13, 2014

Area of Science:

  • Molecular Biology
  • Systems Biology
  • Biophysics

Background:

  • mRNA decay is crucial for cellular adaptation and gene expression.
  • Existing methods for measuring mRNA lifetimes have limitations, including transcription inhibition and separate experiments for transcription rate estimation.

Purpose of the Study:

  • To develop a method for simultaneously determining mRNA transcription rates and lifetimes.
  • To utilize regulatory small RNAs (sRNAs) to control mRNA decay and enable simultaneous measurements.

Main Methods:

  • Analysis of a stochastic model for coupled degradation of mRNAs and sRNAs.
  • Derivation of exact results connecting RNA lifetimes and transcription rates to mean abundances.
  • Generalization of results to include nonstoichiometric coupled degradation of sRNAs.

Main Results:

  • Established a theoretical framework for simultaneous determination of mRNA transcription and decay rates.
  • Provided a method to analyze the efficiency of stoichiometric regulation by sRNAs.
  • Identified parameters influencing mRNA transcription and decay processes.

Conclusions:

  • The proposed approach offers a more accurate and integrated method for studying gene expression dynamics.
  • This framework facilitates the analysis of regulatory mechanisms involving small RNAs.
  • Suggests experimental protocols for precise parameter determination in RNA regulation studies.