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Multiple luteinizing hormone/chorionic gonadotropin receptor messenger ribonucleic acid transcripts.
H Wang1, M Ascoli, D L Segaloff
1Department of Physiology and Biophysics, University of Iowa, Iowa City 52242.
Endocrinology
|July 1, 1991
Summary
The luteinizing hormone/choriogonadotropin (LH/CG) receptor exhibits diverse mRNA transcripts across species and cell types. Despite variations in mRNA, the cell surface receptor size remains consistent, though one transcript may not yield functional protein.
Area of Science:
- Molecular Endocrinology
- Genomics
- Cell Biology
Background:
- Multiple messenger RNA (mRNA) species for the luteinizing hormone/choriogonadotropin (LH/CG) receptor are found in gonadal tissues.
- Previous studies identified LH/CG receptor mRNA using specific probes.
Purpose of the Study:
- To investigate the variability in LH/CG receptor mRNA transcript sizes and abundance across different species and cell types.
- To determine if variations in mRNA transcripts affect the size of the expressed cell surface receptor.
Main Methods:
- Comparative analysis of LH/CG receptor mRNA using specific probes in rat, mouse, and transfected human embryonic kidney 293 cells.
- Examination of LH/CG receptor mRNA in MA-10 Leydig tumor cells.
- Assessment of cell surface receptor size.
Main Results:
- Significant variation in the sizes and relative abundance of LH/CG receptor mRNA transcripts was observed between rat and mouse gonadal tissues.
- Transcript patterns differed between species and also from human embryonic kidney 293 cells transfected with rat LH/CG receptor cDNA.
- Despite mRNA diversity, the cell surface LH/CG receptor size was identical in rat, mouse, and human embryonic kidney 293 cells.
- MA-10 cells predominantly express a 1.2 kilobase LH/CG receptor mRNA transcript encoding a truncated, extracellular hormone-binding domain.
Conclusions:
- Species-specific variations in LH/CG receptor mRNA exist, but do not alter the final cell surface receptor size.
- A common truncated LH/CG receptor mRNA transcript in MA-10 cells is likely not translated into a functional protein, suggesting post-transcriptional regulation or alternative functions.