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Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Real-time Analyses of Retinol Transport by the Membrane Receptor of Plasma Retinol Binding Protein
14:32

Real-time Analyses of Retinol Transport by the Membrane Receptor of Plasma Retinol Binding Protein

Published on: January 28, 2013

Fluorescence-based technique for analyzing retinoic acid.

Leslie J Donato1, Noa Noy

  • 1Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 17, 2010
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel optical method to measure retinoic acid (RA) concentrations in biological samples. This technique utilizes a modified cellular retinoic acid-binding protein I (CRABP-I) and fluorescence detection for sensitive RA quantification.

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Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases
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Published on: January 28, 2013

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases
07:22

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

Published on: March 11, 2016

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Retinoic acid (RA) is a crucial transcriptional activator involved in development and tissue maintenance.
  • RA exhibits potent anticarcinogenic properties and is used in cancer therapy.
  • Accurate measurement of RA concentrations in biological samples is essential for research and clinical applications.

Purpose of the Study:

  • To develop a novel optical method for quantifying retinoic acid (RA) concentrations in biological samples.
  • To utilize cellular retinoic acid-binding protein I (CRABP-I) as a specific and high-affinity binder for RA detection.
  • To enable sensitive and reliable measurement of RA levels in various biological matrices.

Main Methods:

  • Engineered a variant of cellular retinoic acid-binding protein I (CRABP-I) by replacing Leucine-28 with Cysteine.
  • Covalently attached an environmentally sensitive fluorescent probe to the engineered CRABP-I.
  • Measured changes in fluorescence intensity upon binding of RA to the modified CRABP-I protein.
  • Validated the method for measuring RA in serum and monitoring its biosynthesis and degradation in cultured cells.

Main Results:

  • The developed method allows for reliable measurement of RA concentrations as low as 50 nM.
  • Demonstrated effective application of the method for quantifying RA in human serum samples.
  • Successfully monitored the dynamic processes of RA biosynthesis and degradation in cultured mammalian cells.

Conclusions:

  • The novel optical method provides a sensitive and specific approach for measuring RA concentrations.
  • This technique has significant potential for applications in clinical diagnostics and biological research.
  • The method facilitates a deeper understanding of RA's role in physiological and pathological processes.