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Updated: Jun 12, 2026
Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds
Published on: June 13, 2018
Self-assembly-peptide hydrogels as tissue-engineering scaffolds for three-dimensional culture of chondrocytes in
Jingping Liu1, Hong Song, Lanlan Zhang
1Nanomedicine Laboratory, West China Hospital, Institute for Nanobiomedical Technology and Membrane Biology, West China Hospital, Sichuan University, Chengdu, P. R. China.
Abstract:
The promising potential of a RAD-16 self-assembly-peptide hydrogel as a scaffold for tissue-engineered cartilage was investigated. Within 3 weeks of in vitro culture, chondrocytes within the hydrogel produced a high amount of GAG and type-II collagen, which are the components of cartilage-specific extracellular matrix (ECM). With the culture time increased, toluidine-blue staining for GAG and immuno-histochemistry staining for type-II collagen of the chondrocytes-hydrogel composites became more intense. Analysis of the gene expression of the ECM molecules also confirmed the chondrocytes in the peptide hydrogel maintained their phenotype within 3 weeks of in vitro culture.
