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Updated: Jun 12, 2026

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
Published on: July 17, 2021
Population data and sequence analysis of a 'new' microsatellite locus HumHUU (D16S3433)
Magdalena Konarzewska1, Witold Pepinski, A Jagiello
1Department of Medical Genetics, Warsaw Medical University, Warsaw, Poland.
Abstract:
This study reports sequence characteristics and population genetic data on a 'new' STR locus HumHUU (D16S3433) located in the non-coding region of chromosome 16q. Based on a population sample of 306 non-related Polish individuals 205 genotypes and 15 alleles with length range of 157-211bp were distinguished. No deviation from HWE was observed. The sequence analysis of each D16S3433 allele revealed a tetranucleotide repeat motif with a basic sequence structure (AAAA)(0-1)(AAAG)(11-22)(AAAAG)(AAAA)(AG)(AAAAAAG). The power of discrimination is 0.9538, showing a high degree of polymorphism. The presented results demonstrate that the D16S3433 is a useful genetic marker for forensic purposes and paternity testing.

