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Related Concept Videos

Flame Photometry: Overview01:02

Flame Photometry: Overview

Flame photometry, also known as flame emission spectrometry, is a technique used for the qualitative and quantitative analysis of elements present in a sample using a flame as the source of excitation energy. The concept of flame photometry was realized in the early 1860s by Kirchhoff and Bunsen, who discovered that specific elements emit characteristic radiation when excited in flames. The first instrument developed for this purpose was used to measure sodium (Na) in plant ash using a Bunsen...
Flame Photometry: Lab01:16

Flame Photometry: Lab

In a flame photometer, when a solution like potassium chloride is aspirated into the flame, the solvent evaporates, leaving behind dehydrated salt. This salt dissociates into free gaseous atoms in their ground state. Some of these atoms absorb energy from the flame, leading to their excitation. The excited atoms return to the ground state, emitting photons at characteristic wavelengths. Because only electronic transitions are involved, the resulting emission lines are very narrow. The intensity...
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
Photoelectric Effect02:26

Photoelectric Effect

When light of a particular wavelength strikes a metal surface, electrons are emitted. This is called the photoelectric effect. The minimum frequency of light that can cause such emission of electrons is called the threshold frequency, which is specific to the metal. Light with a frequency lower than the threshold frequency, even if it is of high intensity, cannot initiate the emission of electrons. However, when the frequency is higher than the threshold value, the number of electrons ejected...
Microbial Growth Measurement: Direct Methods01:23

Microbial Growth Measurement: Direct Methods

Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...

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Related Experiment Video

Updated: Jun 12, 2026

High-Throughput Analysis of Non-Photochemical Quenching in Crops Using Pulse Amplitude Modulated Chlorophyll Fluorometry
10:08

High-Throughput Analysis of Non-Photochemical Quenching in Crops Using Pulse Amplitude Modulated Chlorophyll Fluorometry

Published on: July 6, 2022

Dayglow photometry: a new approach.

R Narayanan, J N Desai, N K Modi

    Applied Optics
    |June 18, 2010
    PubMed
    Summary

    A novel photometer precisely measures faint daytime thermospheric emissions, overcoming background noise. This breakthrough enables new studies of atmospheric phenomena like dayglow.

    Area of Science:

    • Atmospheric Physics
    • Optical Instrumentation
    • Aeronomy

    Background:

    • Measuring faint atmospheric emissions against bright solar backgrounds is challenging.
    • Previous attempts to measure daytime thermospheric emissions faced significant limitations.

    Purpose of the Study:

    • To develop and commission a unique photometer for measuring daytime oxygen (OI) 630.0-nm emissions in the thermosphere.
    • To overcome the limitations of prior instruments in detecting low-intensity signals.

    Main Methods:

    • Utilized a low-resolution (10^4) Fabry-Perot etalon.
    • Incorporated a temperature-tuned, narrowband (3-Å) interference filter.
    • Employed a radial chopper and an up/down counting system for background elimination.

    More Related Videos

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    Published on: October 27, 2017

    Measurement of Aerosols Optical Thickness of the Atmosphere using the GLOBE Handheld Sun Photometer
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    Measurement of Aerosols Optical Thickness of the Atmosphere using the GLOBE Handheld Sun Photometer

    Published on: May 29, 2019

    Related Experiment Videos

    Last Updated: Jun 12, 2026

    High-Throughput Analysis of Non-Photochemical Quenching in Crops Using Pulse Amplitude Modulated Chlorophyll Fluorometry
    10:08

    High-Throughput Analysis of Non-Photochemical Quenching in Crops Using Pulse Amplitude Modulated Chlorophyll Fluorometry

    Published on: July 6, 2022

    Indoor Experimental Assessment of the Efficiency and Irradiance Spot of the Achromatic Doublet on Glass (ADG) Fresnel Lens for Concentrating Photovoltaics
    09:00

    Indoor Experimental Assessment of the Efficiency and Irradiance Spot of the Achromatic Doublet on Glass (ADG) Fresnel Lens for Concentrating Photovoltaics

    Published on: October 27, 2017

    Measurement of Aerosols Optical Thickness of the Atmosphere using the GLOBE Handheld Sun Photometer
    06:27

    Measurement of Aerosols Optical Thickness of the Atmosphere using the GLOBE Handheld Sun Photometer

    Published on: May 29, 2019

    Main Results:

    • Successfully developed and commissioned a photometer capable of measuring line intensities below 0.1% of the background continuum.
    • Demonstrated the ability to measure daytime OI 630.0-nm emission from the thermosphere.

    Conclusions:

    • The developed photometer overcomes previous measurement limitations for faint atmospheric phenomena.
    • The instrument's design and background elimination method open possibilities for various applications beyond current dayglow studies.