Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flame Photometry: Overview01:02

Flame Photometry: Overview

Flame photometry, also known as flame emission spectrometry, is a technique used for the qualitative and quantitative analysis of elements present in a sample using a flame as the source of excitation energy. The concept of flame photometry was realized in the early 1860s by Kirchhoff and Bunsen, who discovered that specific elements emit characteristic radiation when excited in flames. The first instrument developed for this purpose was used to measure sodium (Na) in plant ash using a Bunsen...
Flame Photometry: Lab01:16

Flame Photometry: Lab

In a flame photometer, when a solution like potassium chloride is aspirated into the flame, the solvent evaporates, leaving behind dehydrated salt. This salt dissociates into free gaseous atoms in their ground state. Some of these atoms absorb energy from the flame, leading to their excitation. The excited atoms return to the ground state, emitting photons at characteristic wavelengths. Because only electronic transitions are involved, the resulting emission lines are very narrow. The intensity...
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which are...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lidar thermometry using two-line atomic fluorescence.

Applied optics·2019
Same author

Development of an alkali chloride vapour-generating apparatus for calibration of ultraviolet absorption measurements.

The Review of scientific instruments·2017
Same author

An Indirect Treatment Comparison of Cabozantinib Verse Vandetanib in Progressive Medullary Thyroid Cancer (MTC).

Value in health : the journal of the International Society for Pharmacoeconomics and Outcomes Research·2016
Same author

Vapor phase tri-methyl-indium seeding system suitable for high temperature spectroscopy and thermometry.

The Review of scientific instruments·2015
Same author

Optical diagnostics of a gliding arc.

Optics express·2013
Same author

Comparison of photo detectors and operating conditions for decay time determination in phosphor thermometry.

The Review of scientific instruments·2012

Related Experiment Video

Updated: Jun 12, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Simultaneous multiple species detection in a flame using laser-induced fluorescence.

U Westblom, M Aldén

    Applied Optics
    |June 18, 2010
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a laser-induced fluorescence method for simultaneously detecting nitric oxide (NO), hydroxyl radicals (OH), and oxygen atoms (O) in flames. The technique utilizes spectral coincidences for precise measurements, aiding combustion analysis.

    More Related Videos

    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
    10:21

    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

    Published on: May 5, 2016

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    Related Experiment Videos

    Last Updated: Jun 12, 2026

    Fluorescence detection methods for microfluidic droplet platforms
    14:16

    Fluorescence detection methods for microfluidic droplet platforms

    Published on: December 10, 2011

    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
    10:21

    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

    Published on: May 5, 2016

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    Area of Science:

    • Chemical Physics
    • Spectroscopy
    • Combustion Science

    Background:

    • Accurate measurement of reactive species like nitric oxide (NO), hydroxyl radicals (OH), and oxygen atoms (O) is crucial for understanding flame chemistry and combustion processes.
    • Traditional methods for detecting multiple species simultaneously in flames can be complex and limited in spatial resolution.

    Purpose of the Study:

    • To present a novel laser-induced fluorescence (LIF) approach for the simultaneous detection of NO, OH, and O in flames.
    • To investigate the feasibility of spatially resolved measurements using a diode-array detector with this LIF technique.
    • To demonstrate the application of this method for studying laser-induced disturbances in flames.

    Main Methods:

    • Utilized a Nd:YAG-based laser system to generate frequency-doubled (287 nm) and frequency-mixed (226 nm) laser beams.
    • Employed laser-induced fluorescence (LIF) based on spectral coincidences for simultaneous species detection.
    • Investigated the use of a diode-array detector for spatially resolved LIF measurements.

    Main Results:

    • Successfully demonstrated simultaneous detection of NO, OH, and O in flames via LIF.
    • Confirmed the capability of the technique for spatially resolved measurements.
    • Showcased the utility of the LIF approach in analyzing laser-induced disturbances.

    Conclusions:

    • The presented LIF technique offers a viable method for simultaneous, spatially resolved detection of key flame species (NO, OH, O).
    • This approach provides a valuable tool for advanced combustion diagnostics and flame chemistry research.
    • The technique's ability to study laser-induced disturbances opens avenues for optimizing laser-based combustion control and analysis.