Related Experiment Video
Updated: Jun 12, 2026
Routine Screening Method for Microparticles in Platelet Transfusions
Published on: January 31, 2018
Platelet-derived microparticles are removed by a membrane plasma separator
Norio Hanafusa1, Hiroshi Satonaka, Kent Doi
1Departments of Hemodialysis and Apheresis, The University of Tokyo Hospital, Japan. hanafusa-tky@umin.ac.jp
Abstract:
Platelet-derived microparticles (PDMPs) are released from activated platelets and are closely related to various diseases. Using the enzyme-linked immunosorbent assay, PDMP concentrations in blood and separated plasma of eight patients who underwent apheresis using membrane plasma separator at our hospital were tested. Considerable amounts of PDMPs were filtrated using plasma separators. However, the concentrations of PDMPs within the blood at the end of the therapy did not differ significantly from those before the session. Plasmapheresis itself might have activated platelets to release PDMPs or perhaps PDMPs within the supplementary fluid increased PDMPs during the therapy. After resolving these points, plasmapheresis could become an effective therapy against elevated PDMP conditions.
Insights
Platelet-derived microparticles (PDMPs) are released from activated platelets. While apheresis filtration removed significant PDMPs, blood levels did not significantly decrease post-therapy, suggesting potential platelet activation during the procedure.
Area of Science:
- Hematology
- Biomedical Engineering
Background:
- Platelet-derived microparticles (PDMPs) are cell-free vesicles released from activated platelets.
- Elevated PDMP levels are associated with various pathological conditions.
Purpose of the Study:
- To investigate the efficacy of membrane plasma separation in removing PDMPs from circulation.
- To assess the impact of apheresis on PDMP concentrations in patients undergoing the procedure.
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) was used to quantify PDMP concentrations.
- Blood and plasma samples from eight patients undergoing membrane apheresis were analyzed.
Main Results:
- Plasma separators significantly filtered considerable amounts of PDMPs.
- However, PDMP concentrations in the blood at the end of apheresis therapy did not significantly differ from pre-therapy levels.
Conclusions:
- Apheresis filtration effectively removes PDMPs, but post-therapy blood concentrations remain unchanged.
- Further research is needed to determine if plasmapheresis activates platelets, increasing PDMP levels, or if supplementary fluids contribute.
- Optimizing plasmapheresis protocols could establish it as a viable therapy for conditions with elevated PDMPs.
