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Routine Screening Method for Microparticles in Platelet Transfusions
Published on: January 31, 2018
Platelet-derived microparticles are removed by a membrane plasma separator.
Norio Hanafusa1, Hiroshi Satonaka, Kent Doi
1Departments of Hemodialysis and Apheresis, The University of Tokyo Hospital, Japan. hanafusa-tky@umin.ac.jp
ASAIO Journal (American Society for Artificial Internal Organs : 1992)
|June 19, 2010
Summary
Platelet-derived microparticles (PDMPs) are released from activated platelets. While apheresis filtration removed significant PDMPs, blood levels did not significantly decrease post-therapy, suggesting potential platelet activation during the procedure.
Area of Science:
- Hematology
- Biomedical Engineering
Background:
- Platelet-derived microparticles (PDMPs) are cell-free vesicles released from activated platelets.
- Elevated PDMP levels are associated with various pathological conditions.
Purpose of the Study:
- To investigate the efficacy of membrane plasma separation in removing PDMPs from circulation.
- To assess the impact of apheresis on PDMP concentrations in patients undergoing the procedure.
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) was used to quantify PDMP concentrations.
- Blood and plasma samples from eight patients undergoing membrane apheresis were analyzed.
Main Results:
- Plasma separators significantly filtered considerable amounts of PDMPs.
- However, PDMP concentrations in the blood at the end of apheresis therapy did not significantly differ from pre-therapy levels.
Conclusions:
- Apheresis filtration effectively removes PDMPs, but post-therapy blood concentrations remain unchanged.
- Further research is needed to determine if plasmapheresis activates platelets, increasing PDMP levels, or if supplementary fluids contribute.
- Optimizing plasmapheresis protocols could establish it as a viable therapy for conditions with elevated PDMPs.
