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Updated: Jun 12, 2026

Immunostaining-Based Detection of Dynamic Alterations in Red Blood Cell Proteins
Published on: March 17, 2023
Modifications of band 3 and oxidation level of membrane proteins in senescent erythrocytes
Angel Rucci1, María Alejandra Ensinck, Nicolas Mufarrege
1Facultad de Ciencias Bioquímicas y Farmacéuticas. Universidad Nacional de Rosario, Argentina.
Objectives:
To study membrane proteins modifications in Senescent Red Blood Cells (SeRBC). DESIGN ANDMETHODS: SeRBC were obtained on Percoll gradients. Membrane proteins were analyzed by SDS-PAGE, band 3 by immunoblotting, and protein oxidation by measuring the carbonyl groups.
Results:
Densitometric analysis showed no change in SeRBC while an increase in band 3 and its degradation products was found. An increase of protein oxidation level was found in SeRBC.
Conclusions:
These findings provide further experimental evidence about protein modifications occurring during the RBC lifespan.
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