Bidirectional immobilization of affinity-tagged cytochrome c on electrode surfaces
Florian Schröper1, Arnd Baumann, Andreas Offenhäusser
1Institute of Bio- and Nanosystems-2, Research Center Jülich, JARA-Fundamentals of Future Information Technology, 52425 Jülich, Germany.
Abstract:
Here, we report a new strategy for the directed bivalent immobilization of cyt c on or between gold electrodes. C-terminal modification with cys- or his-tag did not affect the functional integrity of the protein. In combination with electrostatic protein binding, these tags enable a bifunctional immobilization between two electrodes or alternatively one electrode and interacting enzymes.


