Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

B3GNT2 promotes gastric cancer metastasis by facilitating membrane trafficking of RYK via N-glycosylation.

Biochimica et biophysica acta. Molecular basis of disease·2026
Same author

Comprehensive genomic and computational insights into Brucella suis: pan-genome analysis, evolutionary perspectives, and in-silico vaccine design.

BMC genomics·2026
Same author

Metrnl ameliorates ulcerative colitis by inhibiting NLRP3 inflammasome activation via ASC.

Scientific reports·2026
Same author

m5C and m6A cooperatively stabilize EPHB4 to drive lymphatic metastasis in gastric cancer.

Frontiers in genetics·2026
Same author

Clinical significance and functional characterization of RRN3 in gastric cancer: insights from pan-cancer analysis and experimental validation.

Frontiers in oncology·2026
Same author

The Role and Mechanisms of Tumor-Derived Exosomes in the Formation of the Premetastatic Niche.

Cancer control : journal of the Moffitt Cancer Center·2026

Related Experiment Video

Updated: Jun 12, 2026

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
11:42

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

Published on: December 28, 2015

Rapid protein production using CHO stable transfection pools.

Jianxin Ye1, Krista Alvin, Haythem Latif

  • 1Merck & Co., Inc., Bioprocess Research and Development, Rahway, NJ 07065, USA. jianxin_ye@merck.com

Biotechnology Progress
|June 22, 2010
PubMed
Summary

Stable transfection pools offer a rapid method for producing gram quantities of monoclonal antibodies (mAbs) within two months. This approach achieves high expression levels and is scalable, providing an efficient alternative to transient transfection for preclinical development.

More Related Videos

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
07:09

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

Published on: July 31, 2012

Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach
11:20

Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach

Published on: October 16, 2014

Related Experiment Videos

Last Updated: Jun 12, 2026

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
11:42

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

Published on: December 28, 2015

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
07:09

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

Published on: July 31, 2012

Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach
11:20

Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach

Published on: October 16, 2014

Area of Science:

  • Biotechnology
  • Protein Expression
  • Mammalian Cell Culture

Background:

  • Early preclinical development requires substantial amounts of therapeutic proteins for various studies.
  • Transient transfection is common but often yields insufficient quantities, particularly in Chinese Hamster Ovary (CHO) cells.
  • A need exists for efficient, scalable methods to rapidly produce representative protein materials.

Purpose of the Study:

  • To present stable transfection pool technology as an alternative strategy for rapid protein material generation.
  • To evaluate the yield, scalability, and productivity enhancement of this method for monoclonal antibody (mAb) production.
  • To assess factors influencing productivity, including cell sorting, culture duration, and vector design.

Main Methods:

  • Utilized stable transfection pool technology for monoclonal antibody production.
  • Employed fluorescence-activated cell sorting (FACS) to enrich high-producing cells.
  • Investigated the impact of culture duration and incorporated Universal chromatin-opening elements (UCEs) into expression vectors.

Main Results:

  • Generated gram quantities of mAbs within two months post-transfection.
  • Achieved expression levels ranging from 100 mg/L to over 1000 mg/L.
  • Demonstrated successful scale-up to 200 L using disposable bioreactors; FACS improved productivity threefold; UCEs significantly enhanced productivity; identified an optimal production window.

Conclusions:

  • Stable transfection pools provide a robust and rapid method for producing large quantities of therapeutic proteins.
  • This technology offers high yields and scalability, suitable for early preclinical development needs.
  • Product quality, including glycan distribution, is comparable to that from traditional clonal cell lines.