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Updated: Jun 12, 2026

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
Published on: March 1, 2018
pCEC coupling with ESI-MS for the analysis of beta(2)-agonists and narcotics using a poly-(1-hexadecene-co-TMPTMA)
Jintian Cheng1, Lan Zhang, Qiaomei Lu
1Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, The Sport Science Research Center, Fuzhou University, Fuzhou, P. R. China.
Abstract:
A pressure-assisted CEC with ESI-MS based on poly(1-hexadecene-co-trimethylolpropane trimethacrylate) monolithic column for rapid analysis of two beta(2)-agonists and three narcotics was established in this article. After the organic polymer-based monolithic column was prepared by an in-situ polymerization procedure, a systematic investigation of the pressure-assisted CEC separation and ESI-MS detection parameters was performed. Baseline separation of the studied analytes could be obtained using the solution containing 75% ACN v/v and 20 mmol/L ammonium acetate with pH 8.0 as running buffer, when applying separation voltage of 20 kV and assisted pressure of 5 bar. Under the optimized conditions, two beta(2)-agonists and three narcotics could be completely resolved and accurately determined within 15 min. Finally, the proposed method was successfully used for real urine samples detection.
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