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Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
Published on: July 23, 2013
Reprogramming plant gene expression: a prerequisite to geminivirus DNA replication
Linda Hanley-Bowdoin1, Sharon B Settlage, Dominique Robertson
1Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC 276957622, USA.
Molecular Plant Pathology
|June 23, 2010
Summary
Geminiviruses reprogram plant cells to replicate their DNA by manipulating the retinoblastoma/E2F cell cycle network. This allows viral DNA amplification in mature plant cells lacking essential replication machinery.
Area of Science:
- Plant virology
- Molecular biology
- Cell cycle regulation
Background:
- Geminiviruses are small, single-stranded DNA viruses that infect plants.
- They rely on host DNA polymerases for genome amplification.
- Replication often occurs in differentiated cells with low DNA replication factors.
Purpose of the Study:
- To review how geminiviruses induce DNA replication machinery in mature plant cells.
- To explore the modulation of the retinoblastoma/E2F network by geminiviruses.
- To understand how cell cycle re-entry supports viral genome amplification.
Main Methods:
- Review of recent experimental findings.
- Analysis of geminivirus interactions with host regulatory networks.
- Comparison with mammalian DNA tumor virus replication strategies.
Main Results:
- Geminiviruses reprogram host gene expression to accumulate replication factors.
- They modulate the retinoblastoma/E2F transcription regulatory network.
- This induces quiescent cells to re-enter the cell cycle for viral replication.
Conclusions:
- Geminiviruses hijack the host cell cycle machinery for replication.
- Interactions with regulatory networks influence tissue and host specificity.
- Understanding these mechanisms is key to controlling geminivirus infections.
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