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Related Concept Videos

Subviral Agents01:29

Subviral Agents

Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
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Related Experiment Video

Updated: Jun 12, 2026

Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm
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Beet poleroviruses: close friends or distant relatives?

Mark Stevens1, Benjamin Freeman, Hsing-Yeh Liu

  • 1Broom's Barn Research Station, Higham, Bury St Edmunds, Suffolk IP28 6NP, UK.

Molecular Plant Pathology
|June 23, 2010
PubMed
Summary
This summary is machine-generated.

Three poleroviruses cause sugar beet yellowing: Beet mild yellowing virus (BMYV), Beet chlorosis virus (BChV), and Beet western yellows virus-USA (BWYV-USA). These viruses are identified using RT-PCR, as species-specific antibodies are unavailable.

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Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
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Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Published on: July 27, 2018

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Last Updated: Jun 12, 2026

Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm
11:12

Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm

Published on: April 12, 2017

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
08:56

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Published on: July 27, 2018

Area of Science:

  • Plant Virology
  • Molecular Biology

Background:

  • Three distinct poleroviruses, Beet mild yellowing virus (BMYV), Beet chlorosis virus (BChV), and Beet western yellows virus-USA (BWYV-USA), are responsible for inducing yellowing symptoms in sugar beet.
  • Turnip yellows virus (TuYV) is the new designation for non-beet-infecting isolates of BWYV found in Europe.

Purpose of the Study:

  • To detail the taxonomy, physical properties, host range, symptoms, and transmission of poleroviruses affecting sugar beet.
  • To highlight the diagnostic methods for differentiating between closely related poleroviruses.

Main Methods:

  • Virus identification is achieved through Reverse Transcription Polymerase Chain Reaction (RT-PCR) utilizing specific primers targeting the 5' end of the viral genomes.
  • Host susceptibility was assessed in various Chenopodiaceae species, including commercial sugar beet, red beet, and spinach, along with experimental hosts like *Montia perfoliata*, *Nicotiana benthamiana*, and *Arabidopsis thaliana*.

Main Results:

  • Poleroviruses cause characteristic yellowing (chlorosis) in older sugar beet leaves, leading to thickening and brittleness.
  • The virus particles are isometric, approximately 26 nm in diameter, with a single-stranded positive-sense RNA genome.
  • Transmission occurs persistently via aphid vectors, with *Myzus persicae* identified as a primary vector.

Conclusions:

  • RT-PCR provides a reliable method for distinguishing between BMYV, BChV, and BWYV-USA in the absence of specific antibodies.
  • Understanding the host range, symptoms, and transmission dynamics is crucial for managing polerovirus diseases in sugar beet and related crops.