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Updated: Jun 12, 2026
An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity
Published on: May 12, 2020
Accurate non-invasive image-based cytotoxicity assays for cultured cells
Patricia Marqués-Gallego1, Hans den Dulk, Claude Backendorf
1Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
Background:
The CloneSelect Imager system is an image-based visualisation system for cell growth assessment. Traditionally cell proliferation is measured with the colorimetric MTT assay.
Results:
Here we show that both the CloneSelect Imager and the MTT approach result in comparable EC50 values when assaying the cytotoxicity of cisplatin and oxaliplatin on various cell lines. However, the image-based technique was found non-invasive, considerably quicker and more accurate than the MTT assay.
Conclusions:
This new image-based technique has the potential to replace the cumbersome MTT assay when fast, unbiased and high-throughput cytotoxicity assays are requested.
