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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

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Updated: Jun 12, 2026

Co-localizing Kelvin Probe Force Microscopy with Other Microscopies and Spectroscopies: Selected Applications in Corrosion Characterization of Alloys
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Co-localizing Kelvin Probe Force Microscopy with Other Microscopies and Spectroscopies: Selected Applications in Corrosion Characterization of Alloys

Published on: June 27, 2022

Profilometry with a coherence scanning microscope.

B S Lee, T C Strand

    Applied Optics
    |June 23, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Coherence scanning microscopy offers high-resolution imaging by using coherence effects, enhancing lateral and longitudinal resolution. This technique decouples resolution and improves profilometry measurements.

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    Co-localizing Kelvin Probe Force Microscopy with Other Microscopies and Spectroscopies: Selected Applications in Corrosion Characterization of Alloys
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    The Evolution of Silica Nanoparticle-polyester Coatings on Surfaces Exposed to Sunlight

    Published on: October 11, 2016

    Area of Science:

    • Optical microscopy
    • Metrology
    • Surface characterization

    Background:

    • Confocal microscopy is a standard high-resolution imaging technique.
    • Traditional methods often link lateral and longitudinal resolution.
    • Profilometry requires precise surface measurement.

    Purpose of the Study:

    • Introduce coherence scanning microscopy as a novel high-resolution imaging technique.
    • Explore its application and advantages in profilometry.
    • Detail the method's features and provide illustrative examples.

    Main Methods:

    • Coherence scanning microscopy utilizes coherence effects instead of physical apertures.
    • The technique enhances both lateral and longitudinal resolution.
    • Interference effects are leveraged for improved longitudinal resolution.

    Main Results:

    • Longitudinal resolution is decoupled from lateral resolution.
    • Significant enhancements in longitudinal resolution are achievable.
    • Coherence scanning profilometry demonstrates practical utility through examples.

    Conclusions:

    • Coherence scanning microscopy provides superior resolution in profilometry.
    • The technique offers flexibility by decoupling lateral and longitudinal resolution.
    • This method represents a significant advancement in high-resolution surface metrology.