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Related Experiment Video

Updated: Jun 12, 2026

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

Centromere activity in dicentric small supernumerary marker chromosomes.

Elisabeth Ewers¹, Kinya Yoda, Ahmed B Hamid

¹Institute of Human Genetics and Anthropology, Jena University Hospital, Kollegiengasse 10, 07743, Jena, Germany.

Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology

|June 23, 2010

Summary

This study reveals how centromeric activation patterns in dicentric small supernumerary marker chromosomes (sSMC) vary. Findings suggest gender may influence these patterns, impacting sSMC stability and clinical outcomes.

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Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

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Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System

Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System

Published on: December 21, 2016

Area of Science:

Genetics
Molecular Biology
Cytogenetics

Background:

Dicentric small supernumerary marker chromosomes (sSMC) are complex genetic structures.
Understanding centromeric activity is crucial for their biological characterization.

Purpose of the Study:

To investigate centromeric activation patterns in 25 dicentric sSMC.
To explore factors influencing these patterns, including chromosome origin and carrier gender.
To assess the potential impact on sSMC stability and clinical outcomes.

Main Methods:

Immunohistochemistry using centromere proteins (CENP)-B and CENP-C.
Analysis of sSMC derived from chromosomes #13/21, #14, #15, #18, and #22.
Evaluation of centromeric activity and plasticity.

Main Results:

Three predominant centromeric activation patterns were identified: centric fusion, single active centromere, or dual active centromeres.
A potential influence of carrier gender was observed, with females showing a higher frequency of single active centromeres.
Centromeric distance significantly correlated with activation patterns; distances between 1.4-13 Mb favored dual activity, while >15 Mb led to inactivation.

Conclusions:

Centromeric activation patterns in dicentric sSMC are influenced by chromosome origin, centromere proximity, and potentially carrier gender.
These patterns may affect sSMC stability and mosaicism, contributing to clinical phenotypes.
Further research into mechanisms like meiotic imprinting is warranted.