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Rat monoclonal antibody specific for the chromatin remodeling factor, CHD1

Saori Yoshimura1, Akihito Harada, Jun Odawara

  • 1Department of Bioengineering, Graduate School of Engineering, Osaka City University, Osaka, Japan.

Hybridoma (2005)
|June 24, 2010
PubMed

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Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
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Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...