Related Experiment Video
Updated: Jun 12, 2026
11:10
Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study
Published on: June 29, 2016
Monoclonal antibody specific for Dhx9/NDHII/RHA
Manato Kotani1, Akihito Harada, Jun Odawara
1Department of Bioengineering, Graduate School of Engineering, Osaka City University, Osaka, Japan.
Hybridoma (2005)
|June 24, 2010
Summary
Researchers developed a new monoclonal antibody for Dhx9 (Double-stranded RNA helicase 9). This antibody specifically detects Dhx9 protein and confirms its nuclear localization, aiding future research on RNA metabolism.
Area of Science:
- Molecular Biology
- Protein Biochemistry
- Immunology
Background:
- The DEAH protein family, including Dhx9 (Double-stranded RNA helicase 9), is crucial for RNA metabolism.
- These proteins possess essential domains for RNA binding and helicase activity, impacting transcription regulation.
- Understanding Dhx9's function requires specific tools to study its expression and localization.
Purpose of the Study:
- To establish a highly specific monoclonal antibody for the Dhx9 protein.
- To validate the antibody's utility in detecting Dhx9 and determining its cellular location.
Main Methods:
- Generation of a monoclonal antibody against Dhx9 using the rat medial iliac lymph node immunization method.
- Immunoblot analysis to confirm antibody specificity for full-length Dhx9.
- Immunocytochemical staining to determine the subcellular localization of Dhx9.
Main Results:
- A novel monoclonal antibody specific to Dhx9 was successfully established.
- Immunoblotting confirmed the antibody's ability to detect full-length Dhx9 protein.
- Immunocytochemistry demonstrated that Dhx9 is primarily localized in the cell nucleus.
Conclusions:
- The developed monoclonal antibody is a valuable tool for specific Dhx9 detection.
- This antibody enables further detailed investigations into Dhx9 expression patterns and functions.
- The confirmed nuclear localization provides insights into Dhx9's role in cellular processes.
