Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Efficient spatio-temporal modeling for human action recognition from RGB streams using unitary temporal encoding and adaptive consistency refinement.

Scientific reports·2026

Same author

DNA barcoding and phylogenetic insights into the selected endemic flora of the Western Himalayas.

Scientific reports·2026

Same author

Investigation of lead-free double perovskite Cs₂CeAgCl₆ for resistive switching and optoelectronic applications.

Scientific reports·2026

Same author

Activation of sarco/endoplasmic reticulum Ca²⁺-ATPase 2 (SERCA2) reduces brain injury and improves cognitive function following ischemic stroke.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026

Same author

Letter to Editor on: "Trends and Racial/Ethnic Disparities in Pharmacologic and Surgical Management of Treated Female Patients With Urinary Incontinence".

Neurourology and urodynamics·2026

Same author

Molecular detection of Cyclospora cayetanensis in fresh produce and irrigation water in peri-urban settings: a cross-sectional study of the environmental-agricultural interface.

BMC microbiology·2026

Search research articles

Related Experiment Video

Updated: Jun 12, 2026

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

MRT letter: Optimal composite depth function for 3D shape recovery of microscopic objects.

Abdul Majid, Muhammad Tariq Mahmood, Tae-Sun Choi

Microscopy Research and Technique

|June 24, 2010

Summary

This study introduces an optimal composite depth (OCD) function using genetic programming to improve 3D microscopic object structure estimation. The novel approach enhances depth map accuracy for diverse objects compared to single focus measures.

More Related Videos

3D Scanning Technology Bridging Microcircuits and Macroscale Brain Images in 3D Novel Embedding Overlapping Protocol

3D Scanning Technology Bridging Microcircuits and Macroscale Brain Images in 3D Novel Embedding Overlapping Protocol

Published on: May 12, 2019

Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography

Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography

Published on: October 17, 2018

Related Experiment Videos

Last Updated: Jun 12, 2026

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

3D Scanning Technology Bridging Microcircuits and Macroscale Brain Images in 3D Novel Embedding Overlapping Protocol

3D Scanning Technology Bridging Microcircuits and Macroscale Brain Images in 3D Novel Embedding Overlapping Protocol

Published on: May 12, 2019

Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography

Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography

Published on: October 17, 2018

Area of Science:

Microscopy
Computer Vision
Computational Imaging

Background:

Traditional shape-from-focus techniques rely on single focus measures for 3D reconstruction.
Single focus measures exhibit limitations in accurately estimating depth maps for varied microscopic object types.

Discussion:

A novel genetic programming approach is presented to develop an optimal composite depth (OCD) function.
The OCD function effectively integrates depth and focus information from multiple individual focus measures.
This composite approach overcomes the limitations of single-measure techniques in 3D microscopic imaging.

Key Insights:

The proposed OCD function significantly improves the accuracy of depth map estimation for microscopic objects.
Demonstrated superior performance on both synthetic and real-world microscopic datasets.
Highlights the potential of evolutionary computation in enhancing imaging analysis.

Outlook:

Further refinement of the OCD function could lead to more robust 3D reconstruction in challenging microscopic environments.
Potential applications in fields requiring precise 3D structural analysis, such as cell biology and materials science.
Future work may explore real-time implementation for dynamic microscopic processes.