Simultaneous detection of different serum pepsinogens and its primary application

Area of Science:

• Biochemistry
• Immunology
• Analytical Chemistry

Background:

• Pepsinogens (PGs) are crucial biomarkers for gastric health.
• Accurate and efficient detection of PGs is vital for diagnosing gastric diseases.
• Existing diagnostic methods may lack the desired sensitivity or throughput.

Purpose of the Study:

• To develop a simple, rapid, and sensitive dual-label time-resolved fluoroimmunoassay for human serum pepsinogens.
• To establish a high-throughput method for serological screening of gastric diseases.

Main Methods:

• A two-site sandwich fluoroimmunoassay protocol was employed.
• Monoclonal antibodies against pepsinogen I (PG I) and PG II were co-coated.
• Tracer monoclonal antibodies were labeled with europium (Eu) and samarium (Sm) chelates for dual-label detection.

Main Results:

• The assay demonstrated high sensitivity with detection limits of 0.2 µg/L for PG I and 0.05 µg/L for PG II.
• The assay provided a wide dynamic range for both PG I (5.0-320.0 µg/L) and PG II (1.0-55.0 µg/L).
• Results showed good agreement with single-label assays and ELISA, with average recovery rates of 102.7% for PG I and 98.8% for PG II.

Conclusions:

• The developed dual-label fluoroimmunoassay is a simple, rapid, and sensitive method for quantifying pepsinogens.
• This assay is suitable for high-throughput serological screening of gastric diseases.
• The assay provides reliable results comparable to established methods.