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[RAPD analysis on the germplasm resources of Baphicacanthus cusia]
1Institute of Agrobiological resources, Fujian Academy of Agricultural Sciences/Medicinal Plant Research Center, Fuzhou 350003, China.
Objective:
To explore the genetic diversity of germplasm resources of Baphicacanthus cusia on molecular leve.
Methods:
The molecular biological technique-random amplified polymorphic DNA (RAPD) were used.
Results:
104 random decamer primers were screened for RAPD fragments of Baphicacanthus cusia. 422 DNA bands were amplified by 12 primers, 55.69% products were found to be polymorphic. Base on UPGMA cluster analysis, a DNA molecular dendrogram was established to discuss the genetic diversity of the germplasm resources of Baphicacanthus cusia. The genetic differences are related to morphological differences on a certain extant, but not to geographic regions.
Conclusion:
There actually existed much genetic diversity on molecular level among different natural populations of Baphicacanthus cusia.
