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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Updated: Jun 11, 2026

Pyrosequencing for Microbial Identification and Characterization
12:37

Pyrosequencing for Microbial Identification and Characterization

Published on: August 22, 2013

Single-molecule sequencing: sequence methods to enable accurate quantitation.

Christopher Hart1, Doron Lipson, Fatih Ozsolak

  • 1Helicos BioSciences Corporation, Cambridge, Massachusetts, USA.

Methods in Enzymology
|June 29, 2010
PubMed
Summary
This summary is machine-generated.

Helicos Single-Molecule Sequencing offers unbiased, direct sequencing of nucleic acids for genome biology. This method enables accurate quantitation and variant detection from minimal sample input, advancing research in genomics and diagnostics.

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Last Updated: Jun 11, 2026

Pyrosequencing for Microbial Identification and Characterization
12:37

Pyrosequencing for Microbial Identification and Characterization

Published on: August 22, 2013

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Cost-Efficient Transcriptomic-Based Drug Screening
06:40

Cost-Efficient Transcriptomic-Based Drug Screening

Published on: February 23, 2024

Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • Traditional sequencing methods often involve complex sample preparation, including ligation and amplification steps.
  • Unbiased quantitation and sequence information from cellular and extracellular nucleic acids are crucial for comprehensive genome biology insights.

Purpose of the Study:

  • To describe the methods and applications of Helicos Single-Molecule Sequencing for direct nucleic acid analysis.
  • To highlight the advantages of unbiased, direct sequencing for various genomic applications.

Main Methods:

  • Direct sequencing of DNA and RNA using a flow-cell surface with oligo-dT hybridization.
  • Sample preparation involves shearing, poly-A tailing, and hybridization, avoiding ligation or amplification.

Main Results:

  • Demonstrated applications include human genome sequencing for variant detection, ChIP Seq with picogram DNA quantities, and copy number variation studies.
  • Successfully identified new classes of small RNAs and captured nucleic acids from as few as 400 cells.

Conclusions:

  • Helicos Single-Molecule Sequencing provides a powerful tool for researchers, enabling biological measurements at the single-molecule level.
  • This technology offers significant opportunities for genomic scientists, translational researchers, and diagnostic experts.