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RP-HPLC Method for the Estimation of Dutasteride in Tablet Dosage Form
Dipti B Patel1, N J Patel, S K Patel
1S. K. Patel College of Pharmaceutical Education & Research, Department of Pharmaceutical Chemistry, Ganpat University, Kherva-382 711, India.
Abstract:
A simple, sensitive and precise RP-HPLC method was developed for the determination of dutasteride in tablet dosage form. The RP-HPLC separation was achieved on phenomenex C(18) column (250 mm, id 4.6 mm, 5 mum) using mobile phase methanol:water (90:10 v/v) at a flow rate of 1 ml/min at an ambient temperature. Quantification was achieved with photodiode array detection at 235 nm over the concentration range 1-12 mug/ml. The method was validated statistically and was applied successfully for the determination of dutasteride in tablets.
