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Updated: Jun 11, 2026
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Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
Published on: November 26, 2018
[CD14++CD16- and CD14+CD16+ human monocytes adhesion to endothelial cells].
Tsitologiia
|July 1, 2010
Summary
Monocyte subsets CD14+CD16+ and CD14++CD16- show distinct endothelial adhesion. CD14+CD16+ monocytes adhere more strongly, especially under inflammatory conditions induced by TNF alpha.
Area of Science:
- Immunology
- Cell Biology
Background:
- Monocytes, key immune cells, exist as subsets (CD14++CD16- and CD14+CD16+) with differing surface markers.
- These subsets exhibit unique adhesion molecule and chemokine receptor profiles, suggesting distinct roles in endothelium interaction and tissue migration.
Purpose of the Study:
- To investigate the adhesion capabilities of CD14++CD16- and CD14+CD16+ monocyte subsets to endothelial cells.
- To determine the influence of pro- and anti-inflammatory cytokines on monocyte adhesion.
Main Methods:
- Co-culture of human monocyte subsets (CD14++CD16- and CD14+CD16+) with endothelial cells.
- Assessment of monocyte adhesion in the presence and absence of specific cytokines (TNF alpha, IFN gamma, IL-4).
Main Results:
- CD14+CD16+ monocytes demonstrated significantly higher adhesion to resting endothelial cells compared to CD14++CD16- monocytes.
- Tumor Necrosis Factor alpha (TNF alpha), alone or with other cytokines, markedly increased the adhesion of both monocyte subsets.
- Interferon gamma (IFN gamma) and Interleukin-4 (IL-4) did not independently affect monocyte adhesion.
Conclusions:
- Both CD14++CD16- and CD14+CD16+ monocyte subsets are recruited to inflamed endothelium.
- In non-inflammatory conditions, CD14+CD16+ monocytes exhibit approximately twofold greater adhesion to endothelial cells than CD14++CD16- monocytes.
