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[Spectrophometric analysis of volatile compounds in microorganisms].

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A novel spectrophotometric method rapidly detects microorganisms by analyzing volatile compounds, enabling contactless monitoring of bacterial growth and differentiation of fungal metabolic mutants.

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Area of Science:

Microbiology
Analytical Chemistry
Biochemistry

Background:

Detecting microbial growth and metabolic activity is crucial in various scientific fields.
Existing methods for microbial detection can be time-consuming or require direct contact.
Understanding microbial volatile compound production is key to developing novel detection strategies.

Purpose of the Study:

To develop a rapid, contactless spectrophotometric method for microorganism detection.
To assess the method's capability for monitoring bacterial growth.
To differentiate fungal mutants with altered nitrogen metabolism from wild-type strains.

Main Methods:

A modified spectrophotometric technique was employed.
The method relies on detecting volatile compounds excreted or absorbed by microorganisms.
Contactless monitoring of bacterial growth was performed at concentrations above 10(7) cells/ml.

Main Results:

The spectrophotometric method demonstrated rapid detection of microorganisms.
Contactless monitoring of bacterial growth was achieved.
The method successfully discriminated between wild-type Neurospora crassa and mutants deficient in nitrogen metabolism.

Conclusions:

The modified spectrophotometric method offers a rapid and contactless approach for microbial detection.
This technique is effective for monitoring bacterial growth and analyzing metabolic variations in fungi.
Nitrite and nitrate reductase enzymes, regulated by nit-2 and nit-6 genes, are likely involved in the production of volatile compounds in Neurospora crassa.