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Basolateral ionic permeabilities of macula densa cells.

J Y Lapointe¹, P D Bell, A M Hurst

¹Membrane Transport Research Group, University of Montreal, Quebec, Canada.

The American Journal of Physiology

|June 1, 1991

Summary

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This study investigated ion transport in macula densa cells, finding that chloride conductance significantly influences their basolateral membrane potential. This suggests intracellular chloride activity impacts cell function.

Area of Science:

Renal Physiology
Cellular Electrophysiology
Ion Transport Mechanisms

Background:

Macula densa cells play a crucial role in regulating glomerular filtration rate and renal blood flow.
Understanding the ionic transport pathways in these cells is vital for comprehending kidney function.

Purpose of the Study:

To identify and quantify ion conductances at the basolateral membrane of macula densa cells.
To investigate the influence of basolateral ion concentrations on macula densa cell membrane potential.

Main Methods:

Cortical thick ascending limbs with attached glomeruli were perfused with modified Ringer solutions.
Basolateral ion concentrations (Na+, Cl-, K+) were systematically altered.
Basolateral membrane potential (Vbl) was measured using microelectrodes.

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Main Results:

Reduced basolateral Na+ hyperpolarized Vbl; reduced Cl- or NaCl depolarized Vbl.
Increased basolateral K+ caused a small depolarization.
Cl- conductance constituted the largest portion (59%) of the basolateral conductance.

Conclusions:

The basolateral membrane potential of macula densa cells is highly sensitive to changes in intracellular chloride activity.
Apical Na-K-2Cl cotransporter activity may influence macula densa cell membrane potential via intracellular chloride modulation.