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The HOG pathway dictates the short-term translational response after hyperosmotic shock
Jonas Warringer1, Malin Hult, Sergi Regot
1Department of Cell and Molecular Biology, Lundberg Laboratory, University of Gothenburg, S-40530 Göteborg, Sweden.
Molecular Biology of the Cell
|July 1, 2010
Summary
Yeast cells rapidly adjust protein production via translational control during osmotic stress. This rapid response, faster than gene transcription, fine-tunes cellular adaptation by selectively mobilizing specific mRNAs.
Area of Science:
- Cellular Biology
- Molecular Biology
- Yeast Genetics
Background:
- Cellular adaptation to environmental changes involves complex regulatory mechanisms.
- Environmental stressors, like hyperosmotic shock, trigger rapid cellular responses.
- Understanding gene expression regulation at the translational level is crucial for cellular adaptation.
Purpose of the Study:
- To investigate the translational response of yeast cells to mild hyperosmotic shock.
- To identify specific mRNA populations recruited to ribosomes during osmotic stress.
- To elucidate the role of translational control in cellular adaptation.
Main Methods:
- Isolation of polysomes (mRNA associated with multiple ribosomes).
- Oligonucleotide array analysis of polysomal mRNA.
- Verification of mRNA changes at the protein level using constructs with intact untranslated regions.
- Assessment of the role of stress-activated protein kinases (Hog1, Rck2).
Main Results:
- Translational response (mRNA recruitment to ribosomes) is faster than transcriptional response.
- Specific functional groups of mRNAs, including those for membrane proteins and hexose transporters, are translationally upregulated.
- Cytoplasmic ribosomal protein mRNAs are also translationally mobilized.
- Some transcriptionally induced mRNAs are unexpectedly excluded from polysomes.
- Translational regulation is more dependent on Hog1 and Rck2 than transcriptional regulation for highly osmostress-regulated mRNAs.
Conclusions:
- Translational control plays a significant role in the rapid adaptive response of yeast to osmotic stress.
- Selective mRNA recruitment to ribosomes allows for rapid fine-tuning of protein synthesis.
- This study highlights the importance of post-transcriptional regulation in cellular adaptation.
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