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Published on: September 10, 2018
IgE antibody screening in children. Evaluation of the Phadiatop Paediatric
A W van Toorenenbergen1, A P Oranje, A M Vermeulen
1Department of Clinical Chemistry (Laboratory of Allergology), Erasmus University Hospital, Rotterdam, The Netherlands.
Insights
The Phadiatop Paediatric test effectively screens for IgE antibodies in children. Using sample/reference ratios, it identified atopic disease in 99% of cases, even in children without current symptoms.
Area of Science:
- Pediatric Allergy and Immunology
- Clinical Diagnostics
Background:
- Allergen-specific immunoglobulin E (IgE) antibody testing is crucial for diagnosing allergic diseases in children.
- Accurate and efficient screening tools are needed for early identification of atopic conditions.
Purpose of the Study:
- To evaluate the diagnostic performance of the Phadiatop Paediatric test for IgE antibody screening in children.
- To compare the Phadiatop Paediatric test with a combination of Phadiatop and mixed-food-RAST assays.
Main Methods:
- A total of 200 pediatric serum samples were analyzed.
- Results from the Phadiatop Paediatric test were compared with those from "Phadiatop + mixed-food-RAST".
- Clinical data were retrospectively collected for 171 children.
Main Results:
- The Phadiatop Paediatric test demonstrated high efficiency for IgE antibody screening in young children when interpreted using sample/reference ratios.
- 99% of children with clinically diagnosed atopic disease tested positive.
- 17% of children considered non-atopic based on clinical grounds tested positive with both methods.
Conclusions:
- The Phadiatop Paediatric test is an effective tool for screening IgE antibodies in pediatric populations.
- An atopic immune response, indicated by positive IgE levels, may not always correlate with the presence of current clinical symptoms.
- Interpreting results using sample/reference ratios enhances the utility of the Phadiatop Paediatric test.
Abstract:
With 200 sera from children, results of the Phadiatop Paediatric test were compared with results of the combination "Phadiatop + mixed-food-RAST". The Phadiatop Paediatric test was found to be efficient for IgE antibody screening in young children, when the results of this test were not solely given as "positive" or "negative", but also as "sample/reference-ratio". Clinical documentation could be obtained retrospectively for 171 children and revealed that 99% of the children with atopic disease were found positive in the Phadiatop Paediatric, 17% of the children who were considered non-atopic on clinical grounds, were positive both in the Phadiatop Paediatric and in the combination "Phadiatop + mixed-food-RAST". It is concluded that an atopic immune response is not always associated with current symptoms.
