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Real time optical method for localization of inclusions embedded in turbid media

Anabela Da Silva1, Nadia Djaker, Nicolas Ducros

  • 1CEA, LETI, micro-Technologies for Biology and Healthcare Division, 17 rue des Martyrs, F38054 Grenoble Cedex 9, France. anabela.dasilva@fresnel.fr

Optics Express
|July 1, 2010
PubMed

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.