Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Deconvolution01:20

Deconvolution

Deconvolution, also known as inverse filtering, is the process of extracting the impulse response from known input and output signals. This technique is vital in scenarios where the system's characteristics are unknown, and they must be inferred from the observable signals.
Deconvolution involves several mathematical techniques to derive the impulse response. One common approach is polynomial division. In this method, the input and output sequences are treated as coefficients of...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Wavefront estimation through structured detection in laser scanning microscopy.

Biomedical optics express·2026
Same author

Correction of Background in Fluorescence Correlation Spectroscopy for Accurate Determination of Particle Number.

Biomolecules·2026
Same author

Near-infrared MINFLUX imaging enabled by suppression of fluorophore blinking.

Science advances·2025
Same author

The role of RNA in the nanoscale organization of α-synuclein phase separation.

NAR molecular medicine·2025
Same author

Supramolecular Assembly of Graphene-Polyamine-PdS-CdS Photocatalysts for Synergistically Enhanced and Highly Effective Hydrogen Evolution from Water under Visible Light.

Inorganic chemistry·2025
Same author

Phasor-FLIM and SHG imaging for quantitative analysis of lung cancer autofluorescence.

Computational and structural biotechnology journal·2025
Same journal

Long-term stabilization of intensity-difference squeezing from four-wave mixing in rubidium vapor.

Optics express·2026
Same journal

Robust 3D topography measurement of large-range high-aspect-ratio structures based on dual-domain statistical filtering in SD-OCT.

Optics express·2026
Same journal

Broadband transmissive terahertz metasurface for simultaneous quad-mode OAM multiplexing.

Optics express·2026
Same journal

Leveraging two-dimensional materials for high-sensitivity optical sensors: quasi-bound states in the continuum within hybrid metasurfaces.

Optics express·2026
Same journal

Resolution investigation for dual-spherical-wave optical scanning holographic microscopy: methods and performance.

Optics express·2026
Same journal

Robustness of parallel subnetwork-filtered diffractive deep neural networks.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Jun 11, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Automatic deconvolution in 4Pi-microscopy with variable phase.

Giuseppe Vicidomini1, Roman Schmidt, Alexander Egner

  • 1Max-Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.

Optics Express
|July 1, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces a fast computational method for parametric blind deconvolution to improve 4Pi-microscopy. It enables robust image reconstruction even with imperfect alignment or refractive index variations, aiding non-expert users.

More Related Videos

4D Microscopy of Yeast
12:00

4D Microscopy of Yeast

Published on: April 28, 2019

Generating and Analyzing High-Parameter Histology Images with Histoflow Cytometry
05:22

Generating and Analyzing High-Parameter Histology Images with Histoflow Cytometry

Published on: June 21, 2024

Related Experiment Videos

Last Updated: Jun 11, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

4D Microscopy of Yeast
12:00

4D Microscopy of Yeast

Published on: April 28, 2019

Generating and Analyzing High-Parameter Histology Images with Histoflow Cytometry
05:22

Generating and Analyzing High-Parameter Histology Images with Histoflow Cytometry

Published on: June 21, 2024

Area of Science:

  • Microscopy and Imaging Science
  • Optical Physics
  • Computational Imaging

Background:

  • 4Pi-microscopy enhances axial resolution by doubling the effective aperture using opposing objectives.
  • Raw 4Pi data often contains artifacts like ghost images requiring complex reconstruction.
  • Image reconstruction challenges arise from instrument misalignment and specimen refractive index variations.

Purpose of the Study:

  • To develop a computationally efficient method for robust 4Pi-microscopy image reconstruction.
  • To enable simultaneous estimation of the object and phase function without prior knowledge.
  • To facilitate reliable 4Pi-imaging and automatic image restoration for wider accessibility.

Main Methods:

  • Parametric blind deconvolution algorithm.
  • Computationally fast image reconstruction approach.
  • Verification using synthetic and real microscopy data.

Main Results:

  • Successful simultaneous estimation of object and phase function.
  • Robust performance demonstrated even with challenging imaging conditions.
  • Validation of the method's effectiveness on diverse datasets.

Conclusions:

  • The developed method significantly advances reliable 4Pi-imaging.
  • It offers automatic and robust image restoration, simplifying use for non-experts.
  • This approach overcomes limitations of traditional reconstruction methods.