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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

Updated: Jun 11, 2026

Lensless Fluorescent Microscopy on a Chip

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Lensless wide-field fluorescent imaging on a chip using compressive decoding of sparse objects.

Ahmet F Coskun¹, Ikbal Sencan, Ting-Wei Su

¹Electrical Engineering Department, University of California, Los Angeles, CA, USA.

|July 1, 2010

Summary

This summary is machine-generated.

This study introduces a lensfree fluorescent imaging technique using compressive sampling for ultra-large fields of view. The method achieves high spatial resolution without lenses or scanning, enabling applications in high-throughput analysis.

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Last Updated: Jun 11, 2026

Lensless Fluorescent Microscopy on a Chip

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Published on: August 17, 2011

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

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Published on: August 16, 2012

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Published on: December 14, 2009

Area of Science:

Optics and Photonics
Biomedical Imaging
Computational Imaging

Background:

Traditional microscopy often requires lenses and mechanical scanning, limiting field-of-view and throughput.
Lensfree imaging offers potential for miniaturization and high-throughput analysis.
Compressive sampling enables efficient reconstruction of sparse signals from undersampled data.

Purpose of the Study:

To demonstrate a lensfree fluorescent imaging technique for ultra-large fields of view (>8 cm^2).
To utilize compressive sampling for reconstructing sparse fluorescent sources.
To achieve high spatial resolution without lenses or mechanical scanning.

Main Methods:

On-chip fluorescent excitation via a prism interface with total internal reflection.
Collection of emitted fluorescence through an on-chip fiber-optic faceplate.
Lensless recording on a wide field-of-view opto-electronic sensor array.
Compressive sampling based optimization algorithm for image reconstruction.

Main Results:

Achieved approximately 10 microm spatial resolution over an imaging field-of-view of >8 cm^2.
Demonstrated successful reconstruction of sparse fluorescent sources.
Eliminated the need for lenses and mechanical scanning.

Conclusions:

The developed platform provides a wide-field, lensfree fluorescent imaging solution.
This technique is significant for high-throughput imaging cytometry and rare cell analysis.
Potential applications include micro-array research and other large-area biological sample analysis.