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Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
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Published on: January 3, 2016

Resolution properties of nonlinear optical microscopy.

Naoki Fukutake1

  • 1Materials and Advanced Research Laboratory, Nikon Corporation, 10-1 Asamizodai 1-chome, Sagamihara,Kanagawa 228-0828, Japan. fukutake.naoki@nikon.co.jp

Journal of the Optical Society of America. A, Optics, Image Science, and Vision
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PubMed
Summary

This study unifies the analysis of optical resolution in nonlinear microscopy, including multiphoton-excited fluorescence and Raman scattering. It presents image formation formulas applicable to various nonlinear optical systems for enhanced imaging resolution.

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Area of Science:

  • Nonlinear Optics
  • Optical Microscopy
  • Image Formation

Background:

  • Nonlinear optical microscopy utilizes diverse phenomena like multiphoton-excited fluorescence, harmonic generation, and Raman scattering for advanced imaging.
  • Understanding the resolution properties of these varied nonlinear optical systems is crucial for optimizing imaging performance.
  • Existing formalisms may not comprehensively cover the spectrum of coherent, incoherent, and mixed-coherent phenomena employed in these microscopes.

Purpose of the Study:

  • To analyze and unify the resolution properties of diverse nonlinear optical microscopy systems.
  • To develop a generalized formalism for optical resolution applicable to all nonlinear imaging techniques.
  • To present image formation formulas that describe observed image properties across different nonlinear optical phenomena.

Main Methods:

  • Analysis of resolution properties in nonlinear optical microscopy systems.
  • Development of unified image formation formulas for coherent, incoherent, and mixed-coherent phenomena.
  • Formalism for optical resolution applied to multiphoton-excited fluorescence, harmonic generation, and Raman scattering microscopy.

Main Results:

  • A unified framework for analyzing optical resolution in various nonlinear microscopy techniques is established.
  • Image formation formulas are presented that universally describe image properties across different nonlinear optical effects.
  • The transmission cross-coefficient is shown to vary depending on the specific type of nonlinear system, impacting image formation.

Conclusions:

  • The developed formalism provides a comprehensive understanding of optical resolution in nonlinear microscopy.
  • This unified approach facilitates the optimization and design of advanced nonlinear optical imaging systems.
  • The findings highlight the distinct image formation characteristics governed by the transmission cross-coefficient in different nonlinear systems.