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Updated: Jun 11, 2026

Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells
Published on: May 10, 2019
A "GC-rich" method for mammalian gene expression: a dominant role of non-coding DNA GC content in regulation of
Qian Jia1, HongTao Wu2, XingJun Zhou2
1NCPC R&D Center, Shijiazhuang, 050015, China. qianjiazh@hotmail.com.
Abstract:
High mammalian gene expression was obtained for more than twenty different proteins in different cell types by just a few laboratory scale stable gene transfections for each protein. The stable expression vectors were constructed by inserting a naturally-occurring 1.006 kb or a synthetic 0.733 kb DNA fragment (including intron) of extremely GC-rich at the 5' or/and 3' flanking regions of these protein genes or their gene promoters. This experiment is the first experimental evidence showing that a non-coding extremely GC-rich DNA fragment is a super "chromatin opening element" and plays an important role in mammalian gene expression. This experiment has further indicated that chromatin-based regulation of mammalian gene expression is at least partially embedded in DNA primary structure, namely DNA GC-content.
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