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Further study on polyamine compositions in Vibrionaceae
S Yamamoto1, M A Chowdhury, M Kuroda
1Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Canadian Journal of Microbiology
|February 1, 1991
Summary
Norspermidine, an unusual polyamine, is a key marker for identifying Vibrio and Listonella species. Gas-liquid chromatography confirmed its presence in most Vibrio and all Listonella, aiding bacterial classification.
Area of Science:
- Microbiology
- Bacterial Taxonomy
- Biochemistry
Background:
- Unusual polyamines, like norspermidine, are found in Vibrio species.
- The distribution of polyamines in Vibrionaceae and related bacteria requires further investigation.
Purpose of the Study:
- To determine the cellular polyamine composition of various Vibrionaceae family members and related species.
- To evaluate the utility of norspermidine as a taxonomic marker for Vibrio and Listonella.
Main Methods:
- Gas-liquid chromatography (GLC) was employed to analyze cellular polyamine content.
- A nitrogen-selective detector was used for sensitive detection of norspermidine.
Main Results:
- Norspermidine was identified as a major triamine in 23/24 Vibrio species and all 4 Listonella species.
- Listonella species exhibited polyamine profiles indistinguishable from Vibrio species.
- Shewanella putrefaciens and Aeromonas species lacked norspermidine, while Plesiomonas shigelloides profiles resembled Escherichia coli.
Conclusions:
- Norspermidine presence is a valuable generic marker for the identification and classification of Vibrio and Listonella species.
- The developed GLC method offers rapid and sensitive detection of cellular norspermidine.