Related Experiment Video
Updated: Jun 11, 2026
Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype
Published on: May 19, 2023
Endothelial cell transdifferentiation of human glioma stem progenitor cells in vitro
Yaodong Zhao1, Jun Dong, Qiang Huang
1Department of Neurosurgery, the 2nd Affiliated Hospital of Soochow University, No. 1055 Sanxiang Street, Suzhou, Jiangsu 215004, PR China.
Abstract:
The transdifferentiation of normal stem cells in many kinds of tissues or organs has been studied. However, whether glioma stem cells can transdifferentiate is seldom reported. Meanwhile, the mechanism of angiogenesis in tumors is in disputations, and it is still unknown that whether glioma stem/progenitor cells (GSPCs) participate into angiogenesis in glioma. In this study, we cultivated GSPCs in endothelial differentiation medium for 10 days and found they present to be the typical "flagstone" appearance of vascular endothelial cell (VEC); when cultured on Matrigel, GSPCs gradually formed tubular-like structures in vitro, and cells, which formed the tubular-like structures, showed similar ultrastructural characteristics of VEC under a transmission electron microscope. Furthermore, when cultured in hypoxia or oxygen-glucose deprivation for 4h, GSPCs transcribed and expressed molecular markers of VEC, including CD31, CD34 and vWF. These results indicated that GSPCs could participate into angiogenesis of glioma by transdifferentiating into VEC-like cells.
More Related Videos
10:28Flow Cytometry-based Drug Screening System for the Identification of Small Molecules That Promote Cellular Differentiation of Glioblastoma Stem Cells
Published on: January 10, 2018
12:25Optimization of High Grade Glioma Cell Culture from Surgical Specimens for Use in Clinically Relevant Animal Models and 3D Immunochemistry
Published on: January 7, 2014