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Updated: Jun 11, 2026

Identification of Plasmodesmal Localization Sequences in Proteins In Planta
Published on: August 15, 2017
Expression of epitope-tagged proteins in plants
1Research Institute for Bioresources, Okayama University, Okayama, Japan.
Abstract:
Although immunoelectron microscopy is a powerful tool for visualizing the subcellular localization of target proteins, it is difficult to obtain and purify the specific antibodies required for this method. Instead of raising antibodies against individual target proteins, the use of transgenic plants expressing epitope-tagged proteins and commercially available antibodies simplifies the subcellular localization of target proteins. In this chapter, an improved method for producing transgenic plants that express epitope-tagged proteins and can be used for immunoelectron microscopic analysis is described.
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