Related Experiment Video
Updated: Jun 11, 2026
A Method for Obtaining Serial Ultrathin Sections of Microorganisms in Transmission Electron Microscopy
Published on: January 17, 2018
Serial section immunoelectron microscopy of algal cells
Tetsuaki Osafune1, Steven D Schwartzbach
1Department of Life Science, Nippon Sport Science University, Yokohama, Japan.
This study introduces serial section immunogold electron microscopy to create 3D cell images. This technique visualizes intracellular protein distribution in three dimensions, overcoming 2D imaging limitations.
Area of Science:
- Cell Biology
- Microscopy Techniques
- Biochemistry
Background:
- Electron microscopy with immunogold labeling offers 2D protein distribution insights.
- Cells are inherently 3D structures, posing challenges for 2D imaging.
Purpose of the Study:
- To develop a method for reconstructing 3D cellular images from serial electron micrographs.
- To visualize intracellular protein distribution in three dimensions.
Main Methods:
- Serial section immunogold electron microscopy was employed.
- Cells were fixed and embedded for ultrathin sectioning.
- Sections were labeled with antibodies and protein-A gold, imaged via electron microscopy, and reconstructed using image analysis software.
Main Results:
- A method for 3D cellular image reconstruction from serial sections was successfully described.
- Intracellular protein distribution was visualized in three dimensions.
Conclusions:
- Serial section immunogold electron microscopy enables 3D reconstruction of cellular structures.
- This technique provides a comprehensive view of protein localization within cells.
More Related Videos
10:52Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques
Published on: June 1, 2016
09:58Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy (SEM)
Published on: January 7, 2019
