[Analysis of the circulating immune complexes in experimental lens-induced uveitis]

The authors isolated the circulating immune complexes in experimental lens-induced uveitis by PEG-6000 precipitation and molecular filtration in two peaks. ELISA showed that Peak I was a fraction of antigens comprising four components of soluble lens crystallin, of which gamma-crystallin was the most abundant, but no soluble antigens of the retina; Peak II was the antibodies corresponding to Peak I. SDS electrophoresis demonstrated the presence of over 10 antigen-antibody complexes. The results proved that the toxic complexes of experimental lens-induced uveitis was the lens crystallin immune complexes, and further suggested that gamma-crystallin immune complexes induced experimental uveitis as well as the alpha- and beta-crystallin immune complexes.