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Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
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Related Experiment Video

Updated: Jun 11, 2026

Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle
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Non-carious cervical lesions: a scanning electron microscopic study.

J A Michael1, J A Kaidonis, G C Townsend

  • 1School of Dentistry, The University of Adelaide, South Australia, Australia.

Australian Dental Journal
|July 8, 2010
PubMed
Summary
This summary is machine-generated.

Scanning electron microscopy (SEM) revealed diverse microscopic features of non-carious cervical lesions (NCCLs). This study offers insights into the complex causes of these dental defects.

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Area of Science:

  • Dental Morphology
  • Microscopy
  • Oral Pathology

Background:

  • Non-carious cervical lesions (NCCLs) have a complex etiology that can be clarified using scanning electron microscopy (SEM).
  • This study investigated the morphological characteristics of NCCLs in extracted human anterior teeth.

Purpose of the Study:

  • To examine the diverse morphological forms of non-carious cervical lesions.
  • To utilize scanning electron microscopy for detailed ultrastructural analysis of NCCLs.

Main Methods:

  • Examined 40 non-carious cervical lesions from eight morphologic categories.
  • Utilized scanning electron microscopy at magnifications from 10x to 2605x.
  • Replicated and analyzed five examples per category.

Main Results:

  • Observed a wide range of qualitative ultrastructural features within the NCCLs.
  • Classified observed features based on their microscopic appearance.
  • Identified a total of 13 distinct qualitative features across the lesion sample.

Conclusions:

  • Scanning electron microscopy effectively revealed numerous microscopic features of NCCLs.
  • The findings provide valuable insights into the multifactorial etiology of non-carious cervical lesions.