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Blister fluid immunofluorescence in a case of pemphigus vulgaris

C Shanmugasekar1, V R Ram Ganesh, Alamelu Jayaraman

  • 1Department of Dermatology, PSG Hospitals, Coimbatore, India.

Indian Journal of Dermatology
|July 8, 2010
PubMed

Keywords:
Blisterimmunofluorescencepemphigus

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Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...