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Updated: Jun 11, 2026

Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging
Published on: July 9, 2008
Dynamics of dendritic cell-T cell interactions: a role in T cell outcome
1Department of Pathology, University of Geneva Medical School, Geneva, Switzerland. Stephanie.hugues@unige.ch
Abstract:
Antigen-specific dendritic cells (DC)-T cell encounters occur in lymph nodes (LNs) and are essential for the induction of both priming and tolerance. In both cases, T cells are rapidly activated and proliferate. However, the subsequent outcome of T cell activation depends on the modulation of different DC- and T cell-intrinsic signals. Recent advances in two-photon (2P) microscopy have furthered our understanding regarding the complex choreography of DCs and T cells in intact LNs, and established differences in the dynamics of DC-T cell contacts during priming and tolerance induction. The mechanisms that favour DC-T cell encounters, as well as the contribution of the frequency and the duration of such encounters in dictating the T cell response, are discussed in this review.
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